what happend if air bubble is present on a slide
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How can I get rid of air bubbles trapped under coverslip for 8 chamber slide?
We culture cells in 8 chamber slides and do immunofluorescent staining. We put one drop of mounting media on each of the 8 wells and drop the coverslip at a 45 degree angle. Does anyone have tips on how to get rid of air bubbles trapped between the coverslip and the slide?
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Air bubbles (unless they are too large) are not always a big problem in wet mounts. If larger parts of the specimen are submerged in water, then these parts can be viewed without problems. Air bubbles may cause some problems, especially for beginning microscopists. ... Air bubbles can limit the movement of ciliates.
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