Question

What happens if pKa is lower than pH?

Answer 1

Answer:

If the pH of solution is less than the pKa, the group is in the conjugate acid form (protonated). (Note: glycine can serve as a buffer in 2 different buffer ranges). The isoelectric point (pI) is the pH at which the net charge on the ampholyte is zero (or equal number of + and – charged ions).