Question

What happens when we place the plasmid which lose trtracycline resistance due to insertional inactivation in ampicillin medium?

Answer 1

Hey there.!

when a bacterium containg a plasmid which has lost tetracycline resistance is placed in amicillin medium, Nothing happens to the bacterium.


Reason: Due to insertional inactivation, only tetracycline resistance will be lost but not ampicillin resistance.

This kind of situation is possible when the plasmid is treated with the following enzymes

-BamH1
-Sal1


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