What if you forget to add serum in DMEM while culturing cells?
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Be aware that Serum Free Medium is also a "label" for many cell medium providers (like Life technologies SFM series) meaning that you can culture the cell without adding FBS. This is mainly for easing protein purification by preventing the huge amount of albumins in the classical FBS containing media.
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Serum is commonly used as a supplement to basal growth medium in cell culture. The most common type of serum is fetal bovine serum (FBS) because of its high content of embryonic growth promoting factors. ... Serum also adds buffering capacity to the medium and binds or neutralizes toxic components.
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