Question

What is a cloning vector ? Describe any two features that are required to facilitate cloning into a vector.

Answer 1

Heya..!


cloning vector (vehicle DNA):

these are DNA molecules which can carry a foreign DNA segment and can replicate inside a cell.

SOME FEATURES REQUIRED TO FACILITATE CLONING
1) origin of replication
2) selectable markers
3)cloning sites (recognition sites)


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Answer 2

Cloning Vectors

The DNA molecule that can carry foreign DNA segment and replicate inside the host cell is called vector.

The vectors that may bre used in genetic engineering include;

• Plasmids

• Bacteriophages

• Yeast Artificial Chromosome ( YACs )

• Bacterial Artificial Chromosome ( BACs )

• Cosmids

• Animal and Plant Viruses

Plasmids and bacteriophages are most cmmonly used vectors.

A suitable cloning vector is choosen and cut with a restriction enzymes.

The desired gene is inserted into the cleaved vector by using DNA ligase.

The resulting recombinant vector is then introduced into a host cell for gene manipulation.