what is a good negative control in lactose experiment?
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In negative control, the lacZYAgenes are switched off by repressor when the inducer is absent (signalling an absence of lactose). ... When inducer is present (signalling the presence of lactose), it binds the repressor protein, thereby altering its conformation, decreasing its affinity for o, the operator.
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The lac operon is under both negative and positive control. ... The repressor can bind to a short sequence of the lac promoter. When repressor binds to that sequence it physically blocks access by RNA polymerase to the DNA. As a result, as long as it is bound no lac mRNA can be initiated.
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