Biology, asked by hayama87511, 11 months ago

What is a spreader in leucocyte count and it's characteristics?

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Answered by maheshch989
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Answer:Basic Haematological Techniques

Carol Briggs, Barbara J. Bain, in Dacie and Lewis Practical Haematology (Twelfth Edition), 2017

Manual differential leucocyte count

Differential leucocyte counts are now usually performed, at least on essentially normal samples, by automated instruments. They can also be performed by visual examination of blood films that are prepared on slides by the spread or ‘wedge’ technique. Unfortunately, even in well-spread films, the distribution of the various cell types is not totally random (see below).

For a reliable differential count on films spread on slides, the film must not be too thin and the tail of the film should be smooth. To achieve this, the film should be made with a rapid movement using a smooth glass spreader. This should result in a film in which there is some overlap of the red cells, diminishing to separation near the tail, and in which the white cells in the body of the film are not too

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