What is amplified by Taq polymerase in PCR?
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- Taq DNA polymerase is isolated from Thermus aquaticus and catalyzes the primer-dependent incorporation of nucleotides into duplex DNA in the 5′→3′ direction in the presence of Mg2+. The enzyme does not possess 3′→5′ exonuclease activity but has 5′→3′ exonuclease activity.
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