Question

What is an accurate way to record enzyme activity?

Answer 1

Answer:

Explanation:

The enzymatic activity could be determined and measured by various biochemical tests.

The general idea of these experiments is providing the substrate that the enzyme in question catalyzes to generate a product,

In addition to the substrate, the molecule of a reporter is added to the reaction tube that allows measuring the concentration of the substrate or the product in the real-time.

The reporter could be a compound that changes its color (colorimetric approach), to emit light ( luminescent approach) or fluorescence.

Taking multiple measurements of the change in the reporter emission will provide sufficient data to calculate the enzyme activity.

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Answer 2

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Enzyme kinetics is the study of chemical reactions.

Which is carried out on enzymes by the catalyst.

In enzyme kinetics, the rate of reaction is measured and the reaction of different conditions of effect is investigated.

Studies in the kinetics of an enzyme can reveal in this way the catalytic mechanism of this enzyme, its role in metabolism, how its activity is regulated, and how a drug or an agonist may inhibit the enzyme.