what is anchored pcr
Answers
A variation of the PCR technique, similar to ligation-mediated PCR, that is applied to double-stranded DNA fragments for which the sequence at only one end of the gene is known. The technique allows amplification of a complete sequence of a gene when only the N-terminal sequence of a protein is known.
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Answer:
PCR (polymerase chain reaction) usually requires two specific primers to amplify the DNA bracketed by the two primers. In many circumstances one wishes to characterize unknown sequences contiguous to a known segment of DNA and thus two specific primers are not available. Strategies to overcome this limitation have been developed by adding an anchor to the unknown gene segments and amplifying with the anchor and a specific primer. This paper describes two such techniques, the first in which an anchor is added to cDNA by terminal deoxynucleotidyl transferase, and the second in which an anchor is ligated onto double-stranded DNA.