Question

What is anther cuture ?​

Answer 1

Answer......

☆☆ ANTHER CULTURE ☆☆

(i) The young flower buds are collected and washed under running tap water to remove the dirt’s.

(i) The young flower buds are collected and washed under running tap water to remove the dirt’s.(ii) These are then surface sterilized by immersing in 70% ethanol or sodium hypo­chlorite soln.

(i) The young flower buds are collected and washed under running tap water to remove the dirt’s.(ii) These are then surface sterilized by immersing in 70% ethanol or sodium hypo­chlorite soln.(iii) Then washed in sterile water and transferred into a sterile petridish.

(i) The young flower buds are collected and washed under running tap water to remove the dirt’s.(ii) These are then surface sterilized by immersing in 70% ethanol or sodium hypo­chlorite soln.(iii) Then washed in sterile water and transferred into a sterile petridish.(iv) With the help of sharp scalpel and using forceps the buds are split open and anther lobes are taken out.

(i) The young flower buds are collected and washed under running tap water to remove the dirt’s.(ii) These are then surface sterilized by immersing in 70% ethanol or sodium hypo­chlorite soln.(iii) Then washed in sterile water and transferred into a sterile petridish.(iv) With the help of sharp scalpel and using forceps the buds are split open and anther lobes are taken out.(v) One of the anther lobes of each bud is checked by crushing into acetocarmine stain under microscope for the proper stage of microspore development.

(i) The young flower buds are collected and washed under running tap water to remove the dirt’s.(ii) These are then surface sterilized by immersing in 70% ethanol or sodium hypo­chlorite soln.(iii) Then washed in sterile water and transferred into a sterile petridish.(iv) With the help of sharp scalpel and using forceps the buds are split open and anther lobes are taken out.(v) One of the anther lobes of each bud is checked by crushing into acetocarmine stain under microscope for the proper stage of microspore development.(vi) The filament portions are removed from the selected anther lobes.

(i) The young flower buds are collected and washed under running tap water to remove the dirt’s.(ii) These are then surface sterilized by immersing in 70% ethanol or sodium hypo­chlorite soln.(iii) Then washed in sterile water and transferred into a sterile petridish.(iv) With the help of sharp scalpel and using forceps the buds are split open and anther lobes are taken out.(v) One of the anther lobes of each bud is checked by crushing into acetocarmine stain under microscope for the proper stage of microspore development.(vi) The filament portions are removed from the selected anther lobes.(vii) The damaged anther lobes should be discarded and intact anther lobes are placed into proper media.

(i) The young flower buds are collected and washed under running tap water to remove the dirt’s.(ii) These are then surface sterilized by immersing in 70% ethanol or sodium hypo­chlorite soln.(iii) Then washed in sterile water and transferred into a sterile petridish.(iv) With the help of sharp scalpel and using forceps the buds are split open and anther lobes are taken out.(v) One of the anther lobes of each bud is checked by crushing into acetocarmine stain under microscope for the proper stage of microspore development.(vi) The filament portions are removed from the selected anther lobes.(vii) The damaged anther lobes should be discarded and intact anther lobes are placed into proper media.(viii) Incubated at 24°-28°C in dark for 3-8 weeks.

Answer 2

HEYA MATE.....

YOUR ANSWER IS HERE.........

Anther culture is a technique by which the developing anthers at a precise and critical stage are excised aseptically from unopened flower bud and are cultured on a nutrient medium where the microspores within the cultured anther develop into callus tissue or embryoids that give rise to haploid plantlets either through

#ARMY....