What is batch culture?Describe different phases of growth in a batch culture.
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Answer:
Batch culture is initiated by the transfer of a small portion of a culture into a new culture medium, resulting in growth and an increase in biomass. Biomass concentrationcan be measured in many ways, as cell number, dry weight, packed cell volume, or in terms of any convenient biochemicalcomponent or parameter. The rate of increase in biomass concentration is generally expressed by the specific growth rate (μ).
The changes in specific growth rate during the development of a culture shows the increase of biomass concentration (x) with time (t) in such a batch culture. They indicate that the following phases can be distinguished in the growth of a batch culture: (1) lag phase, (2) accelerating phase, (3) logarithmic phase (balanced growth), (4) decelerating phase, (5) stationary phase, (6) death phase. Each growth phase is a reflection of a particular metabolic state of the cell population at any given time.