What is callus??????
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Answer:
Thickened layers of skin, often on the hands or feet, where friction repeatedly occurs
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Callus:- In this type culture when an explant is placed on a medium gel With agar many of the cells become meristematic l and begin to divide and giving rise to callus in 23 weeks.
Some important points:-
1. The agar medium contains both type of growth regulators auxin like 2,4-D and cytokinin like BAP.
2. Because of the oxidation process these cells may dead as well.
3. Development of those cells can be done by providing required nutrients.
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