Question

What is callus in tissue culture

Answer 1

Plant callus (plural calluses or calli) is a growing mass of unorganized plant parenchyma cells. In living plants,callus cells are those cells that cover a plant wound.

Answer 2

Plant callus (plural calluses or calli) is a growing mass of unorganized plant parenchyma cells. In living plants, callus cells are those cells that cover a plant wound. In biological research and biotechnology callus formation is induced from plant tissue samples (explants) after surface sterilization and plating onto tissue culture medium in vitro (in a closed culture vessel such as a Petri dish).[1] The culture medium is supplemented with plant growth regulators, such as auxins, cytokinins, and gibberellins, to initiate callus formation or somatic embryogenesis. Callus initiation has been described for all major groups of land plants.