what is chromatography?
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★ Chromatography :
- Tsvet was the first to use the term chromatography.
- Chromatography is derived from two Greek words "Chroma" meaning colour and "graphien" meaning to write.
- Chromatography is the science which studies the separation of molecules based on differences in their structure and composition.
- Chromatography is based on the principle where molecules in mixture applied onto the surface or into the solid, and fluid stationary phase is separating from each other while moving with the aid of a mobile phase.
- The separation is based on differential partitioning between the mobile and stationary phases.
- Subtle differences in a compound's partition coefficient result in differential retention on the stationary phase and thus affect the separation.
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Chromatography is the technique used for separation of the components of a mixture that are soluble in the same solvent. This technique was first used for separation of colours, so this name was given. There are two phases in chromatography - the stationary phase and the mobile phase. The components of the mixture are absorbed differently on the stationary phase, thus leading to their separation.
There are many different types of chromatography - paper chromatography, thin layer chromatography, gas chromatography etc. You will study about them in higher grades.
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