what is chromatography?.
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Chromatography
The process of separation of dissolved components of a mixture by adsorbing them over an appropriate adsorbent material is called chromatography. This technique is used for separation of solutes that dissolve in the same solvent. Adsorption is adhesion of atoms, molecules or ions of gas, liquid or solid to a surface.
The name originated from the Greek words kroma (colour) and graphein (to write). Chromatography was first used by the Russian Scientist Mikhail Tsvet in 1903 to separate colourful plant pigments.
1. Adsorbent :
It is a substance that adsorbs another. The adsorbent medium is generally magnesium oxide, alumina, silica gel or filter paper.
2. Adsorbate:
The substance that is adsorbed on the adsorbent is called adsorbate.
3. Solvent.
The solvent generally used for dissolving a mixture of two or more constituents is water or alcohol.
The different constituents of a mixture get adsorbed differently on the same adsorbent material because they have different rates of movement. The rate of movement of adsorbed material depends on:
i) The relative solubility of the constituents of the mixture (adsorbates) in a given solvent.
ii) The relative affinity of the constituents of the mixture for the adsorbent.
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