What is chromatography?
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a technique for the separation of a mixture by passing it in solution or suspension through a medium in which the components move at different rates
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Chromatography is a process of separation of similar substances from their mixture solution.
Explanation:
- Chromatography has been defined as primarily a separation process which is used for the separation of essentially molecular species. It is a physical method of separation.
- It is based on the principle of selective distribution of the components of a mixture between two phases- a Stationary phase(SP) and Mobile Phase(MP). The SP may be solid or liquid while MP is liquid or gas.
- In this method mixture of similar substances are passed through a column or along a plane where the substance is continuously exchanged and equilibrated between two phases.
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