Question

What is chromatography? What are it's various applications and write basic principle involved in brackets.

Answer 1

It is defined as the process of separation of the individual components of a mixture based on their relative affinities towards stationary and mobile phases.

Principle: The samples are subjected to flow by mobile liquid onto or through the stable stationary phase. The sample components are separated into fractions based on their relative affinity towards the two phases during their travel.

The fraction with greater affinity to stationary layer travels slower and shorter distance while that with less affinity travels faster and longer.

Answer 2

it is defined as when your mother is separate butter from milk. As you see she was stearing the milk