Question

WHAT IS CHROMOTOGRAPHY AND WRITE ITS PRINCIPLES AND APPLICATIONS

Answer 1

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Definition & Principle in chromatography. It is defined as the process of separation of the individual components of a mixture based on their relative affinities towards stationary and mobile phases. Principle: The samples are subjected to flow by mobile liquid onto or through the stable stationary phase.

Chromatography is used for quality analyses and checker in the food industry, by identifying and separating, analyzing additives, vitamins, preservatives, proteins, and amino acids. Chromatography like HPLC is used in DNA fingerprinting and bioinformatics.

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