what is cloning vector with example?
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ligation dephosphorylation dna presiptent end repair p c r cloning and more
eg: plasmids ,cosmids,(Bacs) bacterial artificial chromosome ,yeast artificial chromosome(yacs) etc
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A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes. The cloning vector may be DNA taken from a virus, the cell of a higher organism, or it may be the plasmid of a bacterium
Two types of vectors are most commonly used: E. coli plasmid vectors and bacteriophage λ vectors. Plasmid vectors replicate along with their host cells, while λ vectors replicate as lytic viruses, killing the host cell and packaging the DNA into virions
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