Question

what is correct order of staining reagent in gram staining​

Answer 1

Answer:

The performance of the Gram Stain on any sample requires four basic steps that include applying a primary stain (crystal violet) to a heat-fixed smear, followed by the addition of a mordant (Gram's Iodine), rapid decolorization with alcohol, acetone, or a mixture of alcohol and acetone and lastly, counterstaining with

Answer 2

Answer:

Gram staining is a widely used analytical technique used in microbial science to differentiate between the two strains of bacteria i.e. gram positive and gram negative bacteria. This technique was developed by Christian Gram. The correct order of gram staining is:

Heat-fixed smear---crystal violet---gram's iodine---alcohol or acetone---safranine.

Explanation:

In this protocol various sorts of chemical stains are used in a fixed order.

Firstly, the heat-fixed smear of bacterial cells is treated with crystal violet (primary stain) for 2 minutes then it is washed with water and air dried, after this it is treated with Gram's iodine (mordant) for 1 minute and again washed with water and air-dried. Then decolorizing agents like alcohol or acetone are used and the smear is treated with the alcoholic solution and then it is air dried. At last, safranine is used to stain the smear for 30 seconds.

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