Question

what is DNA fingerprinting? what are the steps involved in DNA fingerprinting​

Answer 1

Answer:

There are many steps involved in DNA fingerprinting. The first step is isolating the desired DNA which can be performed chemically, mechanically or enzymatically. The second step is cutting the DNA into several pieces by restriction enzymes. The third step is sorting the DNA pieces by gel electrophoresis

Answer 2

Explanation:

DNA fingerprinting is a technique that shows the genetic makeup of living things. It is a method of finding the difference between the satellite DNA regions in the genome.

STEPS INVOLVED

1. Isolating the DNA.
2. Digesting the DNA with the help of restriction endonuclease enzymes.
3. Separating the digested fragments as per the fragment size by the process of electrophoresis.
4. Blotting the separated fragments onto synthetic membranes like nylon.
5. Hybridising the fragments using labelled VNTR probes.

    6.Analysing the hybrid fragments using autoradiography.

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