what is elution? Name the dye used in gel electrophoresis
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Ethidium bromide dye
Ethidium bromide dye is used to stain the DNA fragment and exposed to UV radiation. DNA bands are then separated and cut out from the agarose gel. After that these bands are extracted from the gel piece. This step is known as elution.
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Elution is generally a process of extracting one material from another by washing with a solvent.
“A dye used to monitor the migration of DNA into a gel or during gel electrophoresis is known as DNA gel loading dye.” Loading dye is an important component in agarose gel electrophoresis. The loading dye comprises bromophenol blue, Ficoll 400 and water majorly while Xylene cyanol, Tris and EDTA are optional in it.
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