What is fixation of the sample in microscopy used for?
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Fixation - serves to "fix" or preserve cell or tissue morphology through the preparation process. ... Thin sections (slices) of material such as tissue may also be applied to a microscope slide for observation. Staining - application of stain to a sample to color cells, tissues, components, or metabolic processes.
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The purpose of fixation is to preserve tissues permanently in as life-like a state as possible. Fixation should be carried out as soon as possible after removal of the tissues (in the case of surgical pathology) or soon after death (with autopsy) to prevent autolysis.
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