what is gene cloning..*
Answers
Answer:
The DNA containing the target genes is segregated into fragments with the help of restriction enzymes. These fragments are then inserted into the cloning vectors, namely the bacteriophages or bacterial plasmids that transfer the recombinant DNA to appropriate host cells; one such is the bacterium E.coil. On the other hand, the complementary DNA is inserted into the naked DNA fragments or vectors that can be taken up by the bacterium directly from its medium
Explanation:
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Cloning is the replication of parent DNA or in other sense amplification of a DNA template and generating many copies of it. The DNA compositions of both the copies and the parent will be exactly the same.
Cloning of any DNA fragment steps :-
- Choice of host organism and cloning vector
- Preparation of vector DNA
- Preparation of DNA to be cloned
- Creation of recombinant DNA
- Introduction of recombinant DNA into host organism