what is gene synth? why is important for us?
Answers
Answer:
Gene synthesis refers to chemically synthesizing a strand of DNA base-by-base. Unlike DNA replication that occurs in cells or by Polymerase Chain Reaction (PCR), gene synthesis does not require a template strand. ... Gene synthesis technologies have revolutionized biology research.
Gene synthesis allows high-throughput, fast, accurate construction of mutant. sequences that are then cloned into expression vectors. Gene synthesis also. facilitates high-level protein expression through host cell-specific codon. optimization, allowing rapid purification of sufficient quantities of protein for.
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Answer:
Gene synthesis is a kind of technique that has been used to synthesize double standard DNA molecules in vitro by artificial way, which is different from single standard oligo nucleotides synthesis in term of syntesis length.
It is important for us as it has emerged as a new application of genetic engineering, utilizing oligonucleotides and different methods of assembling these to generate stretches of double stranded DNA usually cloned into a plasmid vector.