what is Gram's staining?explain?
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Gram staining is a common technique used to differentiate two large groups of bacteria based on their different cell wall constituents. ... Gram positive bacteria stain violet due to the presence of a thick layer of peptidoglycan in their cell walls, which retains the crystal violet these cells are stained with.
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The grouping is based on the reaction of bacteria to Gram's stain.
So basically Gram's staining is all about staining bacteria firstly with crystal violet or gentian violet and then treating them with iodine solution followed by washing with water and alcohol.
Bacteria which retain bluish-violet stain are Gram(+) bacteria while which do not , Gram(-) bacteria.
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