What is holding temperature for nutrient agar?
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agar is substance which provide gelling and thinking to the algae. it provides nourishment.
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Nutrient Agar Plates Preparation & Equipment Use
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Materials:
Nutrient Agar in plastic petri dishes
Inoculation LoopNote: Keep sterile petri dishes closed until ready to pour agar into them.
Air-borne contaminants can easily invade an open petri dish.
Background Information:
Agar is pronounced awger (sounds like foggerwithout the f). Agar is a
gelling agent extracted from red seaweed. Nutrient agar is a commonly
used food medium for microbial cultures. Nutrient agar contains:beef extract (provides carbohydrates, nitrogen, vitamins, salts) peptone (helps control pH) agar (a carbohydrate used as a solidifying agent)
distilled water (an agent for distributing food materials to
growing colonies of micro-organisms)Storage: Stack agar plates upside down in the refrigerator. Do Not Freeze! The purpose of placing the plates upside down is to prevent condensation from dripping down onto the agar surface which could then facilitate movement of organisms between colonies.
Setting Up Your Lab Area:
Set up your lab in an un cluttered area.Keep air movement to a minimum. Air movement can bring unwanted airborne bacteria or molds into your petri dish when you have it open. Do not have a fan, air conditioner, or heater blowing air near your work area. Even the movement of other people or pets can move the air around you. Hold your breath while inoculating the agar or wear a nose and mouth mask.Keep your lab notepad close to record procedures and data.
Preparing the Plates
If plates have been refrigerated, set them out and allow them to warm to room temperature.Sterilize the loopTo sterilize the loop, hold the handle with a pot holder and place the tiny looped wire in a flame until it turns bright redAllow the loop to cool for 3 - 5 seconds before touching the collection area.Resterilize the loop after each inoculation.Do not allow the loop to touch any surface other than the collection area and the agar.Uncover each agar plate just long enough to inoculate the medium. hold the petri dish lid directly over the petri dish (or tilt the lid just enough to allow the loop inside) while inoculating the medium to help prevent contamination from air-borne particles. Do not allow the loop to touch the petri dish.Do not Dip the loop in the agar, let it glide over the surface.Make a pattern of inoculation lines (parallel lines, tic-tac-toe, zig-zag, initials, etc.) to help determine that what is growing is what you put there and not an air-borne contaminant.Place the cover back on the plate immediately.
Incubation:
Place each petri dish inside a zip lock bag to prevent drying out and to control odors.Turn the plates upside down and put them in a warm place. The ideal temperature for incubation is 32° C or 90° F. Bacterial growth should start to become visible in about 2 -3 days.
Science Stuff offers free product consultation, personal customer service,
and a "no strings, satisfaction guaranteed" policy.
Materials:
Nutrient Agar in plastic petri dishes
Inoculation LoopNote: Keep sterile petri dishes closed until ready to pour agar into them.
Air-borne contaminants can easily invade an open petri dish.
Background Information:
Agar is pronounced awger (sounds like foggerwithout the f). Agar is a
gelling agent extracted from red seaweed. Nutrient agar is a commonly
used food medium for microbial cultures. Nutrient agar contains:beef extract (provides carbohydrates, nitrogen, vitamins, salts) peptone (helps control pH) agar (a carbohydrate used as a solidifying agent)
distilled water (an agent for distributing food materials to
growing colonies of micro-organisms)Storage: Stack agar plates upside down in the refrigerator. Do Not Freeze! The purpose of placing the plates upside down is to prevent condensation from dripping down onto the agar surface which could then facilitate movement of organisms between colonies.
Setting Up Your Lab Area:
Set up your lab in an un cluttered area.Keep air movement to a minimum. Air movement can bring unwanted airborne bacteria or molds into your petri dish when you have it open. Do not have a fan, air conditioner, or heater blowing air near your work area. Even the movement of other people or pets can move the air around you. Hold your breath while inoculating the agar or wear a nose and mouth mask.Keep your lab notepad close to record procedures and data.
Preparing the Plates
If plates have been refrigerated, set them out and allow them to warm to room temperature.Sterilize the loopTo sterilize the loop, hold the handle with a pot holder and place the tiny looped wire in a flame until it turns bright redAllow the loop to cool for 3 - 5 seconds before touching the collection area.Resterilize the loop after each inoculation.Do not allow the loop to touch any surface other than the collection area and the agar.Uncover each agar plate just long enough to inoculate the medium. hold the petri dish lid directly over the petri dish (or tilt the lid just enough to allow the loop inside) while inoculating the medium to help prevent contamination from air-borne particles. Do not allow the loop to touch the petri dish.Do not Dip the loop in the agar, let it glide over the surface.Make a pattern of inoculation lines (parallel lines, tic-tac-toe, zig-zag, initials, etc.) to help determine that what is growing is what you put there and not an air-borne contaminant.Place the cover back on the plate immediately.
Incubation:
Place each petri dish inside a zip lock bag to prevent drying out and to control odors.Turn the plates upside down and put them in a warm place. The ideal temperature for incubation is 32° C or 90° F. Bacterial growth should start to become visible in about 2 -3 days.
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