Question

What is important condition for clump formation?

Answer 1

Answer:

Therefore organism that you are using may not be able to tolerate such a high concentration leading to the formation of clumps. In the meanwhile, the effect that you observe with increasing concentration of cyanopyridine could be an indirect effect on the cells by media composition itself.

Answer 2

Answer:

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• The concentration of Fe and Mn used is very high (ie) 100mg/l each which is not very common in the environment.
• Therefore organism that you are using may not be able to tolerate such a high concentration leading to the formation of clumps.
• In the meanwhile, the effect that you observe with increasing concentration of cyanopyridine could be an indirect effect on the cells by media composition itself.
• May be cyanopyridine by itself is also toxic to the bacterial cell at higher concentration that you work with.

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