Question

What is “Insertional Inactivation”?
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Answer 1

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» $\mathscr\pink{Insertional \:Inactivation }</p><p>$ is a procedure, in which a bacteria carrying recombinant plasmids or a fragment of foreign DNA is made to insert into a restriction site inside a gene to resist antibiotics, hence causing the gene to turn non-functional or in an inactivated state.

» The original sequence of disease causing gene is changed and has a proper sequence of required gene (DNA fragment inserted) with high copy number.

Answer 2

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Insertional inactivation is a technique used in recombinant DNA technology. In this procedure, a bacteria carrying recombinant plasmids or a fragment of foreign DNA is made to insert into a restriction site inside a gene to resist antibiotics, hence causing the gene to turn non-functional or in an inactivated state.

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