What is isoelectric point in proteins?
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In proteins the isoelectric point (pI) is defined as the pH at which a protein has no net charge. When the pH > pI, a protein has a net negative charge and when the pH < pI, a protein has a net positive charge. The pI varies for different proteins.
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The isoelectric point (pI) is the pH of a solution at which the net charge of a protein becomes zero. For this reason isoelectric point precipitation is most often used to precipitate contaminant proteins rather than the target protein.
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