What is isoelectric point in proteins?
Answers
Explanation:
=> Isoelectric point is that point of PH at which amino acids have both positive and negative charge in a equal amount.
=> Isoelectric point is a specific value of PH at which amino acids move neither towards cathode nor towards anode.
Answer:
Explanation:
Protein purification includes a series of processes to isolate a particular protein from a complex mixture. This technique is critical for characterizing the function, structure, and interaction of the protein of interest. An analytical purification generally uses three distinct properties to separate proteins, including the isoelectric technique.
protein purification, protein isoelectric pointA protein can be purified according to its protein isoelectric point by running the said protein through an ion exchange column or a pH-graded gel. At the isoelectric point, a protein has no net charge. Above the isoelectric point, a protein carries a net negative charge—below it, a net positive charge.