what is mean by tissue culture
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Tissue culture is the growth of tissues or cells separate from the organism. This is typically facilitated via use of a liquid, semi-solid, or solid growth medium, such as broth or agar. Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows, M.D.
In modern usage, tissue culture generally refers to the growth of cells from a tissue from a multicellular organism in vitro. These cells may be cells isolated from a donor organism, "primary cells", or an immortalised cell line. The cells are bathed in a culture medium, which contains essential nutrients and energy sources necessary for the cells' survival.[8] The term tissue culture is often used interchangeably with cell culture
The literal meaning of tissue culture refers to the culturing of tissue pieces, i.e. explant culture.
Tissue culture is an important tool for the study of the biology of cells from multicellular organisms. It provides an in vitro model of the tissue in a well defined environment which can be easily manipulated and analysed.
Plant tissue culture in particular is concerned with the growing of entire plants from small pieces of plant tissue, cultured in medium[9]
In modern usage, tissue culture generally refers to the growth of cells from a tissue from a multicellular organism in vitro. These cells may be cells isolated from a donor organism, "primary cells", or an immortalised cell line. The cells are bathed in a culture medium, which contains essential nutrients and energy sources necessary for the cells' survival.[8] The term tissue culture is often used interchangeably with cell culture
The literal meaning of tissue culture refers to the culturing of tissue pieces, i.e. explant culture.
Tissue culture is an important tool for the study of the biology of cells from multicellular organisms. It provides an in vitro model of the tissue in a well defined environment which can be easily manipulated and analysed.
Plant tissue culture in particular is concerned with the growing of entire plants from small pieces of plant tissue, cultured in medium[9]
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The procedure in which a small tissue pieces or organs is removed from a donor plant and cultured aseptically on a nutrient medium is called Tissue Culture. This production is also known as micro propagation technique.
A cell or tissue is transferred into suitable synthetic medium under sterile conditions. The tissue then develops into a fast growing cellular mass called callus. The callus is transferred to another medium for growth and differentiation that forms plantlets. The plantlets can be transplanted into soil or pots where they can be grown to maturity.
Tissue culture technique is being popularly used for production of ornamental plants like Orchids, Dahlia and Carnation. Other examples are Banana, Fig, Lemon, Potatoes, etc.
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