what is meaning of chromotography..
Answers
- Chromatography is the separation of components in a mixture.
- The mixture is dissolved in the mobile phase, which then transports it through the stationary phase.
- The mixture's various components move through the stationary phase at different rates, causing them to separate.
- Which substances go more swiftly or slowly, and how they are divided, depends on the characteristics of the mobile and stationary phases.
- Retention time refers to the difference in travel times.
Chromatography is a technique used to separate components, solutes from a solution, elements from a mixture of solution. There is a Stationary phase and a mobile phase through which the separation of elements takes place.
Explanation:
Chromatography is one of the commonly used techniques for separation in chemical and biological fields.
Chromatography is a technique used to separate components, solutes from a solution, elements from a mixture of solution. There is a Stationary phase and a mobile phase through which the separation of elements takes place.
The stationary phase can be a solid or a liquid medium whereas the mobile phase can be a liquid or a gaseous medium. Kinetic molecular motion is the separating force acting on the medium. The medium chosen is solute specific, hence favors the movement of the solute particles along the stream, The other molecules are retained by the stationary phase making its movement slow when compared to the targeted elements.
The various types of chromatography are:
- Column chromatography
- Circular paper chromatography
- Ion- Exchange chromatography
- High performance liquid chromatography
- Affinity chromatography