Question

what is meant by aveena curvature test as in harmone​

Answer 1

Author information: (1)Botanisches Institut der Universität des Saarlandes, Saarbrücken. The Avena geo-curvature test is a bioassay for auxin-type growth regulators. Etiolated oat coleoptiles are used and the test is conducted in special perspex trays under diffuse daylight.

Answer 2

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• A quantitative method used to correlate the degree of curvature obtained in a plant part, after the application of an external stimulus, with the amount of endogenous growth substance required to bring it about.
• The tips of Avena coleoptiles are used as a standard. These are removed and placed on an agar block and auxin allowed  to diffuse out for a predetermined time. The auxin-impregnated agar is then used to measure the curvature produced when pieces are placed acen-trally on decapitated coleoptiles.
• The response is plotted against the auxin concentration in the block. Over a certain range, increases in auxin concentration result in a regular increase in the degree of bending in the coleoptile, but the linear response is lost at higher levels.
• Only the straight line section of the graph is useful for assays. By noting the angle of curvature produced by a given stimulus under otherwise controlled standard conditions, one can find the quantity of auxin involved in producing the response.

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