what is meant by chromatography ? describe with activity paper chromatography
Answers
Answer:
Chromatography is a method for separating the parts of a mixture of either a gas or liquid solution containing different chemicals. For example, pen inks are often made up of different colours. The different bonding properties of each molecule type is exploited. Chromatography is used in both qualitative and quantitative analysis of both organic and inorganic samples.
How Does Paper Chromatography Work?
Chromatography can be demonstrated using a simple demonstration.
What you need:
water soluble pens or markers of different brands or colours
strips of paper towel
water
rubbing alcohol
nail polish remover
straw or pencil or pen
cups
tape
What you do:
Draw a line across 6 paper towel strips or coffee filter strips about 1 inch from the bottom.
Tape the paper towel strip from the end opposite to the pen line onto the middle of the straw so the strip will hang when the straw is placed crosswise onto the cup.
There should be enough water in the cup so that the paper towel strip touches the water but the pen line is NOT submerged.
The strip should not touch the bottom of the cup.
If using alcohol or nail polish remover as all or part of your solvent, place a cap of aluminum foil over the glass to keep the vapours in the glass. It will improve the results.
Observe what happens.
If the pen line remains unchanged in water, try with the same pen type on another strip of paper towel but substitute the alcohol or nail polish remover.
Try mixing one half water and one half alcohol as your solvent and see how the colours separate.
What's Happening in Paper Chromatography?
Ink is a solution containing a number of different molecules. These different molecules have different characteristics such as size and solubility. Solubility is a molecule's ability to dissolve in a particular solvent such as alcohol, water or nail polish remover. Because of their different characteristics, each molecule travels at a different speed when pulled along the piece of paper towelling by the solvent. The lightest particles, which are not necessarily the lightest coloured particles, move more quickly and a greater distance than the heavier particles. Thus, all of the pigments that make up an ink sample are separated out.
Answer:
Chromatography is a method for separating the parts of a mixture of either a gas or liquid solution containing different chemicals. For example, pen inks are often made up of different colours. The different bonding properties of each molecule type is exploited. Chromatography is used in both qualitative and quantitative analysis of both organic and inorganic samples.
How Does Paper Chromatography Work?
Chromatography can be demonstrated using a simple demonstration.
What you need:
water soluble pens or markers of different brands or colours
strips of paper towel
water
rubbing alcohol
nail polish remover
straw or pencil or pen
cups
tape
What you do:
Draw a line across 6 paper towel strips or coffee filter strips about 1 inch from the bottom.
Tape the paper towel strip from the end opposite to the pen line onto the middle of the straw so the strip will hang when the straw is placed crosswise onto the cup.
There should be enough water in the cup so that the paper towel strip touches the water but the pen line is NOT submerged.
The strip should not touch the bottom of the cup.
If using alcohol or nail polish remover as all or part of your solvent, place a cap of aluminum foil over the glass to keep the vapours in the glass. It will improve the results.
Observe what happens.
If the pen line remains unchanged in water, try with the same pen type on another strip of paper towel but substitute the alcohol or nail polish remover.
Try mixing one half water and one half alcohol as your solvent and see how the colours separate.
What's Happening in Paper Chromatography?
Ink is a solution containing a number of different molecules. These different molecules have different characteristics such as size and solubility. Solubility is a molecule's ability to dissolve in a particular solvent such as alcohol, water or nail polish remover. Because of their different characteristics, each molecule travels at a different speed when pulled along the piece of paper towelling by the solvent. The lightest particles, which are not necessarily the lightest coloured particles, move more quickly and a greater distance than the heavier particles. Thus, all of the pigments that make up an ink sample are separated out.
Explanation:
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