What is meant by primary transcript? List three major posttranscriptional modifications for the formation of a mature mRNA from hnRNA.
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The precursor of mRNA transcribed by RNA polymerase-|| it is called heterogeneous nuclear RNA .It undergoes following processing to form nascent mRNA :-
1)Splicing:- In this process the non-coding introns are removed and coding sequences call exons are joined in a definite order .This is required because primary transcript contains both introns and exons .
2)Capping:- In this process an unusual nucleotide is added to the 5' end of hnRNA.
3)Tailing:- In this process 200 to 300 adenylated residues are added at the 3' endof hn RNA .
4)The fully process mRNA is released from the nucleus into the cytoplasm for translation .
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