Question

What is melting of DNA? Describe the concept of Tm value and add a note on factors affecting it.

Answer 1

Answer:

There are two basic approaches to denaturing double-stranded DNA ... Three main factors affect the melting temperature. ... occuring DNAs is summarized in the formula (note: this formula describes the Tm of long DNA hybrids) ... note that the % GC term only holds over a limited range (45 to 75%) as does the salt term ...