what is ment by DNA fingerfrinting?.Explain it in breaf.where this technique is used? give two example
Answers
Answer:
DNA fingerprinting is a laboratory technique used to establish a link between biological evidence and a suspect in a criminal investigation. A DNA sample taken from a crime scene is compared with a DNA sample from a suspect. If the two DNA profiles are a match, then the evidence came from that suspect.
Answer:
it is another name for genetic fingerprinting .
In DNA fingerprinting, fragments of DNA are separated on a gel using a technique called electrophoresis. This creates a pattern that can be analyzed and that is unique to each individual, with the exception of identical twins.
Alec Jeffreys developed this technique in which he used satellite DNAs also called VNTRs (Variable Number of Tandem Repeats) as a probe because it showed the high level of polymorphism.
Following are the steps involved in DNA fingerprinting:
Isolating the DNA.
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Digesting the DNA with the help of restriction endonuclease enzymes.
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Separating the digested fragments as per the fragment size by the process of electrophoresis.
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Blotting the separated fragments onto synthetic membranes like nylon.
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Hybridising the fragments using labelled VNTR probes.
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Analysing the hybrid fragments using autoradiography