What is molecular systematics
Answers
Answer: Molecular systematics is the use of molecular genetics to study the evolution of relationships among individuals and species. The goal of systematic studies is to provide insight into the history of groups of organisms and the evolutionary processes that create diversity among species.
Answer:
PLZ MARK AS BRAINLIEST
Explanation:
Molecular systematics is the use of molecules to determine classification systems and relationships. For hundreds of years botanists used morphology , or overall appearance, to identify and classify plants. Morphological systematics has been important for the basic understanding of plant evolution and relationships; however, it has limitations. One limitation to morphology in plants is homology. Homology assumes that two similar structures have the same evolutionary origin. In other words, the trait arose in an ancestor and was passed down to its descendants. Homology in plant morphology is frequently very difficult to resolve since plant structures can become modified into other forms (e.g., spines of cacti are modified leaves).
Just as a botanist may compare the shape of a leaf between two different plants, molecular systematists compare molecules. Molecules have an advantage over morphology in two aspects. First, homology is usually much easier to determine in molecules than in morphology. Second, molecules tend to provide many more pieces of information than can be gained from morphology. A scientist studying morphology may compare one hundred traits, but a scientist using molecules will compare several hundred to several thousand traits depending on the technique.
Early molecular systematics began with micromolecules. The earliest of these studies can be traced as far back as the 1880s, but much of the work was conducted between the 1950s and 1970s. Micromolecules are small molecules mostly responsible for colors, scents, and chemical defenses of plants. Chemicals found in different plants are identified and compared across species for similarities. Species sharing compounds are presumed to be more closely related. Later botanists used macromolecules, which are proteins and nucleic acids. Much of the work on proteins was conducted in the 1970s and consisted of determining the order of amino acids in specific proteins (protein sequencing) or determining whether different populations or species of plants had different forms of specific enzymes (isozyme variability). Other protein-based studies utilized principles of serology and created antibodies for protein extracts that were compared to extracts from a different species. The degree to which the antibodies of one plant matched the proteins of a another plant provides an estimate of how closely the two plants are related.
Studies began to use deoxyribonucleic acid (DNA) in the late 1960s and 1970s with DNA-DNA hybridization. This method uses the principle that DNA is a double-stranded molecule and that high temperatures (greater than 80°C) can cause all of the DNA to become single-stranded. When cooled, the DNA resumes its double-stranded nature (re-annealling) and the temperature at which it becomes completely double-stranded is an indication of how similar the strands of DNA are. In this method, DNA from two plants is combined and heated. If all of the DNA is from closely related plants, the re-annealling temperature is high. If the DNA is from two distantly related plants it is lower. The re-annealling temperature is an estimate of how similar the plants are. The closer the temperatures are to the re-annealling temperature of a single plant, the more closely the plants are assumed to be related.
During the 1980s botanists made comparisons of DNA between plants using restriction site analysis. Scientists used restriction enzymes that cut DNA into fragments of various lengths. These enzymes cut the DNA at specific combinations of nucleotides every time this combination of sequences is encountered. The fragments are separated by size using gel electrophoresis and visualized by a probe that matches specific regions of the DNA. Comparing fragment sizes, it is possible to determine whether a specific restriction site is present or absent in any given species. The presence of a restriction site in two or more plants implies that the plants with the site have a more recent common ancestor. Restriction site data are capable of producing hundreds of sites depending on the numbers of enzymes that are used. Most botanists use the DNA from the chloroplast since it is smaller in comparison to other regions of the genome and a number of probes are available.