What is partition coefficient of chromatography?
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An analyte is in equilibrium between the two phases; Amobile Astationary. The equilibrium constant, K, is termed the partition coefficient; defined as the molar concentration of analyte in the stationary phase divided by the molar concentration of the analyte in the mobile phase.
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A partition coefficient is the ratio of the concentration of a substance in one medium or phase (C1) to the concentration in a second phase (C2) when the two concentrations are at equilibrium; that is, partition coefficient = (C1/C2)equil. The units of C1 and C2 may be different.
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