What is percentage loading efficiency in micelles and how to calculate
Answers
Answer:
An effective approach that we have used for determining loading and efficiency for Pluronic F127 micelles loaded by the solid dispersion method (common solvent) is (i.) centrifuge drug-loaded micelles (10,000 RCF for 10 min; removes unloaded drug) then lyophilize the supernatant (ii.) extract loaded drug from a known mass of the lyophilized formulation in a suitable organic solvent (e.g., ethanol) at a volume that gives a theoretical drug concentration that ideally fits into the middle of your drug's standard curve (iii.) determine the concentration of drug in the organic solvent (we use LC-MS/MS) and back calculate actual loading (wt%) based on the total mass of the formulation used for drug extraction. Encapsulation efficiency is then actual loading / theoretical loading x 100.