Question

what is primer walking​

Answer 1

Primer walking (or Directed Sequencing) is asequencing method of choice for sequencing DNAfragments between 1.3 and 7 kilobases. Such fragments are too long to be sequenced in a single sequence read using the chain termination method. This method works by dividing the long sequence into several consecutive short ones. The DNA of interest may be a plasmid insert, a PCRproduct or a fragment representing a gap when sequencing a genome. The term "primer walking" is used where the main aim is to sequence the genome. The term "chromosome walking" is used instead when the sequence is known but there is no clone of a gene. For example, the gene for a disease may be located near a specific marker such as an RFLP on the sequence.[1]

Answer 2

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Primer walking (or Directed Sequencing) is a sequencing method of choice for sequencing DNA fragments between 1.3 and 7 kilobases. ... The term "primer walking" is used where the main aim is to sequence the genome.

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