What is recombinant DNA technology
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Recombinant DNA technology is a set of techniques that enables the DNA from different sources to be identified, isolated are recombined so that new characteristics can be introduced into an organism. The farmost technique in recombinant DNA technology is the transfer of a sequence of DNA (gene) from one organism to other organism. Hence, it is also called as Genetic engineering, molecular cloning,or gene cloning. Recombinant DNA technology refers to joining of two (or more) DNAs of different origin to create a new (or novel)DNA molecule. This DNA is transferred to an organism, usually bacteria, where it can multiply using the host machinery.The process of inserting a foreign DNA in a host is also referred as Gene cloning.The cloned gene has to undergo replication, usually independent of the replication of the host genome to make multiple copies. If necessary, regulatory elements are also provided along with the foreign gene. Thus, it is possible to get the foreign gene transcribed and translated and produce the protein coded by the gene. This process is known as expression of cloned gene. A suitable carrier is required to transport the foreign gene to the host cell. This carrier, which is usually an autonomously replicating small DNA molecule, is known as cloning vector. A number of DNA modifying enzymes are needed for the construction of a cassette suitable for gene cloning. These enzymes include the restriction enzymes ligases, polymerases, nucleases etc. If a gene from one species is introduced into another species, that species should produce the same product from that gene. In other words, it should be possible to place a human gene into bacteria and have those bacteria produce the human protein encoded by that gene.infact there are many human genes that produce human proteins in bacteria, and these proteins can be used to treat serious medical problems.
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hope u like my answer!!!
Recombinant DNA technology is a set of techniques that enables the DNA from different sources to be identified, isolated are recombined so that new characteristics can be introduced into an organism. The farmost technique in recombinant DNA technology is the transfer of a sequence of DNA (gene) from one organism to other organism. Hence, it is also called as Genetic engineering, molecular cloning,or gene cloning. Recombinant DNA technology refers to joining of two (or more) DNAs of different origin to create a new (or novel)DNA molecule. This DNA is transferred to an organism, usually bacteria, where it can multiply using the host machinery.The process of inserting a foreign DNA in a host is also referred as Gene cloning.The cloned gene has to undergo replication, usually independent of the replication of the host genome to make multiple copies. If necessary, regulatory elements are also provided along with the foreign gene. Thus, it is possible to get the foreign gene transcribed and translated and produce the protein coded by the gene. This process is known as expression of cloned gene. A suitable carrier is required to transport the foreign gene to the host cell. This carrier, which is usually an autonomously replicating small DNA molecule, is known as cloning vector. A number of DNA modifying enzymes are needed for the construction of a cassette suitable for gene cloning. These enzymes include the restriction enzymes ligases, polymerases, nucleases etc. If a gene from one species is introduced into another species, that species should produce the same product from that gene. In other words, it should be possible to place a human gene into bacteria and have those bacteria produce the human protein encoded by that gene.infact there are many human genes that produce human proteins in bacteria, and these proteins can be used to treat serious medical problems.
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Heyaa folk,
=> It is the study of genetic material.
=> Even scientists are using this technology.
=> The major component of DNA technology is cloning.
Hope it helps u :)
=> It is the study of genetic material.
=> Even scientists are using this technology.
=> The major component of DNA technology is cloning.
Hope it helps u :)
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