What is Recombinant DNA technology? Describe in one sentence each three stages by which this technology is carried out.
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The four steps are: (1) Gene Cloning and Development of Recombinant DNA (2) Transfer of Vector into the Host (3) Selection of Transformed Cells and (4) Transcription and Translation of Inserted Gene.
Knowledge about cell and its functioning has increased to a great magnitude during 20th century. Science and genetics provided basic inputs and now we have gathered information about the structure of master molecule, DNA, its replication and control of gene expression.
Knowledge about cell and its functioning has increased to a great magnitude during 20th century. Science and genetics provided basic inputs and now we have gathered information about the structure of master molecule, DNA, its replication and control of gene expression.
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Recombinant DNA Technology is a method in which two DNA molecules from different species are combined and then inserted into a host species to form a hybrid species, which are beneficial to humans in terms of medicine, agriculture, etc.
Stages of the process:
DNA isolation - With the help of enzymes, DNA molecules are separated from the cell.
DNA fragmentation - genes and vectors are cut using restriction enzymes.
Gene amplification - Polymerase chain reaction (PCR) is performed to amplify the genes and make copies through de-naturalize, anneal, extend.
Host insertion - the recombinant DNA molecule is inserted into the host species using micro-injection, gene gun, etc.
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