What is refolding reactions of proteins slides?
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Protein refolding is an important step in the downstream process of therapeutic proteinproduction when the starting material is an insoluble inclusion body produced by over- expression in E. coli. This is also a step that is least understood in mechanism. Empirical methodologies have been accumulated over the years, and some standard protocols have been established (1). However there are still problems that we encounter when it comes to applying this step in large scale, such as low yield per volume of reaction mixture, low yield of refolded protein, high cost of reagents used, etc.
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Answer:
An optimal refolding condition was achieved by ... for the refolding of immunologically active recombinant proteins ... The reaction was performed by the addition of the ...