Question

what is role of glycerol is used in preparation of competent cells ?

Answer 1

Glycerol prevents the formation of ice crystals when aqueous solutions are frozen. The ice crystals will damage the cellmembranes, killing the cells - so preventing ice crystals is the key to cryopreservation. its a cryopretectant, some ppl woulduse DMSO for mammalian cells.

Answer 2

Glycerol prevents the formation of ice crystals when aqueous solutions are frozen. The ice crystals will damage the cell membranes, killing the cells - so preventing ice crystals is the key to cryopreservation. its a cryopretectant, some ppl would use DMSO for mammalian cells.