What is simple staining
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Simple staining is a method of staining in which bacteria are stained by using a single stain. Simple staining is also called as monochrome staining or positive staining. Examples of simple stain are Methylene blue, Safranin, Malachite green, Basic fuchsin and crystal violet etc.
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In a simple staining technique, a basic, cationic dye is flooded across a sample, adding color to the cells. Before we move on, let's define the word cationic. A cation is simply a positively charged ion. ... This kills the bacteria, attaches the cells to the slide, and enhances the stain uptake.
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