what is spectrophotometry??
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Spectrophotometry is a method to measure how much a chemical substance absorbs light by measuring the intensity of light as a beam of light passes through sample solution. The basic principle is that each compound absorbs or transmits light over a certain range of wavelength.
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A spectrophotometer is an analytical instrument used to quantitatively measure the transmission or reflection of visible light, UV light or infrared light. Spectrophotometers measure intensity as a function of light source wavelength.
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