what is stacking energy
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Stacking (Base Pairs) Regions. ∎ Total free energy of the entire Stacking. Region is given by the addition of each pair of. adjacent base pairs. ∎ This includes energy contributions for both.
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Stacking energies for pair M on N, expressed as TMN, were derived by matrix decomposition of a large set of linear algebraic expressions relating the measured Tm for subtransitions emanating from large polymeric DNAs, and the fractional neighbor frequencies, fMN, for the domains responsible for the transitions
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